Indium 111 - white blood cells

Radiopharmaceuticals

2.Technetium In Vitro Labelling of Red Blood Cells (also includes in-vitro labelling of denatured red blood cells)
(City Hospital, Birmingham)

Method

1. Take 5 ml of blood into a syringe containing approximately 100 units of heparin

2. Transfer 3ml blood into a sterilin vial and add 0.1 ml medronate solution taken from a vial (Amersham N165) reconstituted with 10 ml saline. This is equivalent to 2.6 mgSn(II)

3. Mix and allow to incubate at room temperature for 5 minutes

4. Add 1 ml EDTA solution 4.4% w/v and mix followed by 5 ml saline and mix

5. Centrifuge at 1300 g for 5 minutes. Remove the supernatant with a sterile pipette and discard

6. To the red cell pellet, add the required activity of sodium pertechnetate in a volume of 1 ml. Do not use the first elution of a generator or after weekend storage. For investigation of bleeding sites or cardiac studies, activity is 500-600 MBq. For splenic studies, activity is 120-150 MBq. Where necessary, dilute the pertechnetate to 1 ml with saline

7.
Incubate the technetium red blood cell suspension inside a lead pot at room temperature for 5 minutes with gentle mixing

(For splenic studies incubate 15 minutes at 49.5°C in a water bath with occasional gentle mixing)

8. Draw up the required activity for injection. (For bleeding site- 400 MBq diagnostic reference level; For spleen -100 MBq diagnostic reference level). Replace needle with sterile luer cap.

Labelling Efficiency Determination

To remaining cell suspension add approx 10 ml saline, mix thoroughly, and centrifuge at 1300 g for 5 minutes. Remove the supernant carefully and transfer to another tube. Add 10 ml Water for injection to lyse the sedimented cells. Count the activity in each tube.

Labelling Efficiency = (Activity in lysed cells x 100)/(Activity in lysed cells + activity in supernant)

--------------------------------------

Back to Formulary Section Back to Radiopharmacy Handbook Index

	2.Technetium In Vitro Labelling of Red Blood Cells (also includes in-vitro labelling of denatured red blood cells) (City Hospital, Birmingham)



	Method
1.	Take 5 ml of blood into a syringe containing approximately 100 units of heparin
2.	Transfer 3ml blood into a sterilin vial and add 0.1 ml medronate solution taken from a vial (Amersham N165) reconstituted with 10 ml saline. This is equivalent to 2.6 mgSn(II)
3.	Mix and allow to incubate at room temperature for 5 minutes
4.	Add 1 ml EDTA solution 4.4% w/v and mix followed by 5 ml saline and mix
5.	Centrifuge at 1300 g for 5 minutes. Remove the supernatant with a sterile pipette and discard
6.	To the red cell pellet, add the required activity of sodium pertechnetate in a volume of 1 ml. Do not use the first elution of a generator or after weekend storage. For investigation of bleeding sites or cardiac studies, activity is 500-600 MBq. For splenic studies, activity is 120-150 MBq. Where necessary, dilute the pertechnetate to 1 ml with saline
7.	Incubate the technetium red blood cell suspension inside a lead pot at room temperature for 5 minutes with gentle mixing (For splenic studies incubate 15 minutes at 49.5°C in a water bath with occasional gentle mixing)
8.	Draw up the required activity for injection. (For bleeding site- 400 MBq diagnostic reference level; For spleen -100 MBq diagnostic reference level). Replace needle with sterile luer cap.

	Labelling Efficiency Determination

	To remaining cell suspension add approx 10 ml saline, mix thoroughly, and centrifuge at 1300 g for 5 minutes. Remove the supernant carefully and transfer to another tube. Add 10 ml Water for injection to lyse the sedimented cells. Count the activity in each tube. Labelling Efficiency = (Activity in lysed cells x 100)/(Activity in lysed cells + activity in supernant)




	--------------------------------------