Radiopharmaceuticals

	6: METHOD FOR CONCENTRATING 99mTc GENERATOR ELUATE (For 99mTc-technegas preparation) (Merseyside And Cheshire Radiopharmacy Services).
	Materials Required
	1 x Onguard -AG column (Dionex Ltd)
	1 X BondElut SAX column (Jones Chromatography)
	1 x BondElut adapter (Jones Chromatography)
	Water for injection
	Ethanol
	Sodium Chloride 5% solution
	Nitrogen filled vial
	2 x 21g (green) needles
	1 x 23g (blue) needle
	1 x 1ml syringe
	3 x 5ml syringes
	1ml and 5ml syringe shields
	lead pot
	99mTc generator eluate.
	Method
1.	Wash Onguard-AG column with 5ml water for injections.
2.	Wash BondElut SAX column with 3ml ethanol, followed by 3ml water for injections.
3.	Connect Onguard-AG column to BondElut-SAX column using a BondElut adapter. (MAKE SURE THE CONNECTIONS ARE TIGHT)
4.	Place the lower, BondElut SAX column in a suitable lead shield.
5.	Pass 5ml of 99mTcO4- generator eluate (in a shielded syringe) through the column system, followed by 2ml water for injections. Pass slowly (5ml/min).
6.	Then pass 5ml of air through the system. Use the syringe formerly holding water for injections.
7.	Run effluents to waste.
8.	Disconnect Onguard-AG column and discard.
9.	Fit BondElut-SAX column with green needle (21g). Fit a sterile nitrogen filled vial with a bleed needle (23g) and place in a lead shield.
10.	Pierce septum of N2 vial with BondElut SAX column/needle.
11.	Elute column with 1ml sodium chloride 5%, collecting eluate in vial.
12.	Pass through column 2ml air to ensure maximum extraction of activity.
13.	Calibrate product, label and examine with TLC, if necessary. (Si-gel, n-butanol/acetic acid/water 3:2:3)
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