99mTc(V)-DMSA (Birmingham)

Radiopharmaceuticals

5: ^99mTc-(V) - DMSA (Birmingham City Hospital)

Method.

1. Add 0.17 ml of 4.2% Sodium Bicarbonate solution to an Amersham DMSA kit (N107). This volume is insufficient to dissolve the contents of the vial.

2. Add approximately 500 MBq Sodium pertechnetate in a volume of 3 ml. Equalise the pressure within the vial and shake to dissolve the contents.

3. Measure the activity in the vial and label accordingly. Ensure record sheet is completed with all details.

4. Allow to stand for 15 minutes. During this time, remove a small sample to check pH of the solution using indicator strips. pH should be approximately 8.

5. The final radiopharmaceutical should be a clear colourless solution, devoid of obvious particulate contamination and with a pH of 8.

6. Dose required for patient is 350-400 MBq.

Radiochemical Purity

Two methods are used.

A) TLC Silica Gel (Camlab) in Butanol : Acetic Acid : Water 4:1:1 This takes approx. 1.5 hours to run

Rf values

Tc (III) DMSA 0-0.1

Tc(V) DMSA 0.4-0.5

TcO₄ 0.7-0.8

B) Electrophoresis on Whatman 3MM paper with 0.1M Acetate buffer pH3.6. Run for 300 V for 1 hour

Mobilities cm hr^-1

Tc(III) DMSA 0-0.1

Tc(V) DMSA 6-7

Tc04 10-11

Less than 5% Tc(III) DMSA and less than 5% TcO₄ should be detected.

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	5: ^99mTc-(V) - DMSA (Birmingham City Hospital)

	Method.
1.	Add 0.17 ml of 4.2% Sodium Bicarbonate solution to an Amersham DMSA kit (N107). This volume is insufficient to dissolve the contents of the vial.
2.	Add approximately 500 MBq Sodium pertechnetate in a volume of 3 ml. Equalise the pressure within the vial and shake to dissolve the contents.
3.	Measure the activity in the vial and label accordingly. Ensure record sheet is completed with all details.
4.	Allow to stand for 15 minutes. During this time, remove a small sample to check pH of the solution using indicator strips. pH should be approximately 8.
5.	The final radiopharmaceutical should be a clear colourless solution, devoid of obvious particulate contamination and with a pH of 8.
6.	Dose required for patient is 350-400 MBq.

	Radiochemical Purity
	Two methods are used.

A)	TLC Silica Gel (Camlab) in Butanol : Acetic Acid : Water 4:1:1 This takes approx. 1.5 hours to run
	Rf values
	Tc (III) DMSA	0-0.1
	Tc(V) DMSA	0.4-0.5
	TcO₄	0.7-0.8

B)	Electrophoresis on Whatman 3MM paper with 0.1M Acetate buffer pH3.6. Run for 300 V for 1 hour
	Mobilities cm hr^-1
	Tc(III) DMSA	0-0.1
	Tc(V) DMSA	6-7
	Tc04	10-11
	Less than 5% Tc(III) DMSA and less than 5% TcO₄ should be detected.

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