12-carbon 14-urea solution

Radiopharmaceuticals

12: CARBON - 14 UREA SOLUTION for detection of Helicobacter pylori
(Nottingham University Hospital)

Formula:

¹⁴C Urea solution CFA41 (Amersham Health) 9.25 MBq

Water for injections BP (Phoenix/Antigen/Braun) 100 ml

Administered Dose 45 KBq Orally

Method

The preparation is administered orally. Preparation should be clean, but full aseptic precautions are not required.

1. Using a 10 ml syringe withdraw approx 10 ml water for injections BP from 100 ml bottle.

2. Using this syringe, add approx 1 ml water to a CFA41 vial. Shake gently to dissolve the contents.

3. Using a 2 ml syringe, transfer the contents of the CFA41 vial to the bottle containing the remaining 90 ml of purified water.

4. Wash out the empty CFA41 vial with successive volumes of water from the 10 ml syringe, and transfer the washing to the 100 ml bottle, such that the final volume is 100 ml.

5. Transfer 5 ml aliquots of solution via a 0.22 mm filter into sterilised, sealed vials.

6. Label the vials and store at 2 - 8°C until required.

7. Perform radiochemical purity determination using cellulose with butan-1-ol:water:acetic acid [120:50:30]. Use cold urea (40mg/ml) as marker. After development, identify the position of the radioactivity by autoradiography and that of urea by spraying with 10%p-dimethylamino-benzaldehyde in hydrochloricacid:acetone [1:4]. There should be correspondence in Rf values. Quantification can be achieved by cutting the chromatogram strip into sections, placing each in a vial of liquid scintillant and mixing thoroughly before counting.

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	12: CARBON - 14 UREA SOLUTION for detection of Helicobacter pylori (Nottingham University Hospital)

	Formula:
	¹⁴C Urea solution CFA41 (Amersham Health)	9.25 MBq
	Water for injections BP (Phoenix/Antigen/Braun)	100 ml

	Administered Dose	45 KBq Orally

	Method
	The preparation is administered orally. Preparation should be clean, but full aseptic precautions are not required.

1.	Using a 10 ml syringe withdraw approx 10 ml water for injections BP from 100 ml bottle.
2.	Using this syringe, add approx 1 ml water to a CFA41 vial. Shake gently to dissolve the contents.
3.	Using a 2 ml syringe, transfer the contents of the CFA41 vial to the bottle containing the remaining 90 ml of purified water.
4.	Wash out the empty CFA41 vial with successive volumes of water from the 10 ml syringe, and transfer the washing to the 100 ml bottle, such that the final volume is 100 ml.
5.	Transfer 5 ml aliquots of solution via a 0.22 mm filter into sterilised, sealed vials.
6.	Label the vials and store at 2 - 8°C until required.
7.	Perform radiochemical purity determination using cellulose with butan-1-ol:water:acetic acid [120:50:30]. Use cold urea (40mg/ml) as marker. After development, identify the position of the radioactivity by autoradiography and that of urea by spraying with 10%p-dimethylamino-benzaldehyde in hydrochloricacid:acetone [1:4]. There should be correspondence in Rf values. Quantification can be achieved by cutting the chromatogram strip into sections, placing each in a vial of liquid scintillant and mixing thoroughly before counting.

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